R&D facilities

Atheris laboratories uses modern, state-of-the-art instrumentation and laboratory equipment that is mainly dedicated to biochemistry, protein engineering and analytical chemistry. This is completed by high end informatic equipment for data analysis, to develop and exploit our databases and bioinformatic tools for in silico data- and text-mining, as well as biostatistics.

Fractionation and purification:

• Several HPLC systems (nano, micro, narrow, analytical, semi-preparative and preparative scales).
  
  
• Low pressure preparative chromatographic equipment (gel filtration & ion exchange).
  
  
• Gel electrophoresis (SDS-PAGE & more).
  
  
• Semi-automated solid phase extraction platform (from micro to preparative scales).
  
  
• Centrifuges, Rotavapor, Speed-Vac concentrators, lyophilizers, freezers, etc.
  
  

Mass spectrometry bioanalytics:

• ESI-MS (quadrupole & LC-TOF, coupled on-line to nano or analytical HPLC).
  
  
• MALDI-TOF-MS (with automated spotting on 384-well plates).
  
  
• ESI-MS/MS (triple quadrupole, coupled on line to RP-HPLC).
  
  
• ESI-MS/MS (high resolution Q-TOF, coupled on-line to nano or analytical HPLC).
  
  
• MALDI-TOF-MS/MS (high throughput).
  
  
• Off-line LC-MS and LC-MS/MS.
  
  
• On-line LC-MS and LC-MS/MS (nano to prep, with automated fraction collection).
  
  

Characterization and peptide synthesis:

• Automated Edman gas phase protein sequencing.
  
  
• Solid and liquid phase peptide synthesis (boc & fmoc chemistry).
  
  
• Chemical modifications, labelling with stable isotopes or luminescent dyes.
  
  
• Sample preparation platform for 2D-SDS-PAGE spot proteomic analysis.
  
  

 Bioassays:

• Fully equipped P2 microbiology laboratory.
  
  
• Automated UV-VIS spectrophotometry.
  
  
• Climatic chamber.
  
  
• Proprietary collections of bioactive compound libraries. Visit our Melusine web pages for additional information.